Coding

Part:BBa_K1044001

Designed by: Koki Tsutsumi,Eishin Mitsui   Group: iGEM13_Biwako_Nagahama   (2013-09-05)

beta 1,3 glucan synthase catalytic subunit gene CrdS from Agrobacterium tumefaciens C58.

Curdlan synthesis enzyme requires genes encoded within the CrdS on the C58 linear chromosome. Providing genetic confirmation that crdS is essential for curdlan production. CrdS was shown to be an integral membrane protein with seven transmembrane helices.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal AgeI site found at 1689
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 667
    Illegal BsaI.rc site found at 1415
    Illegal BsaI.rc site found at 1686


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Categories
Parameters
None