Intermediate

Part:BBa_K1042013

Designed by: Parth Patel   Group: iGEM13_Gaston_Day_School   (2013-09-16)

PO promoter + GFP with Stops

We digested the upstream PO Promoter (BBa_I746361) with EcoRI and SpeI and digested the downstream GFP plasmid (BBa_I13401) with XbaI and PstI. These digested fragments were transferred to a Chloramphenicol vector that was cut with EcoRI and PstI. This produced one intermediate that was selectively grown on a Chloramphenicol plate. This intermediate in combination with our other upstream intermediate, the Cadmium Promoter and Ogr Activator (BBa_K1042012) would create a final construct that is theoretically a cadmium sensor with increased sensitivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742


[edit]
Categories
Parameters
None