Composite

Part:BBa_K1040005

Designed by: Taku Tsuzuki   Group: iGEM13_Osaka   (2013-09-17)

RBS(BBa_B0034)+ilvE(BBa_K1040000)

Isoleucine is an essential amino acid and required in the diet by animals. The isoleucine pathway is almost the same as the valine biosynthesis pathway, using the same enzymes, and varying primarily at the first step in the pathway.　Isoleucine biosynthesis begins with the common metabolic intermediate pyruvate, the endpoint of glycolysis. The first step in isoleucine biosynthesis requires thiamine pyrophosphate to form a carbanion intermediate. Another component comes from the amino acid threonine, which is deaminated to produce alpha-ketobutyrate. Alpha-ketobutyrate and the TPP intermediate react to produce alpha-aceto-alpha-hydroxybutyrate that is isomerized, reduced and dehydrated to create alpha-keto-beta-methylvalerate. In the last step, valine aminotransferase transfers an amino group from glutamate to produce isoleucine by branched-chain amino-acid aminotransferase. Branched-chain amino-acid aminotransferase is coded in ilvE. In our project, E. coli we use don't have ilvE and cannot produce isoleucine, an essential amino acid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 162
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRBS(BBa_B0034)+ilvE(BBa_K1040000)