Coding

Part:BBa_K1039029

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-12)


BXB1 integrase with Promoter and RBS

Bxb1 integrase is a serine integrase that catalyzes site-specific recombination between Bxb1 attP (BBa_K1039031) and attB (BBa_K1039032) sites to produce Bxb1 attR (BBa_K1039033) and attL (BBa_K1039034) sites. This recombination is unidirectional, i.e. attR and attL do not recombine in the presence of Bxb1 integrase alone. (Ghaosh et al., 2005). BBa_K1039029 has a promoter and a ribosome binding site of BBa_K608002. In concert with Bxb1 recombination directionality factor (RDF) (BBa_K1039006), Bxb1 integrase catalyzes site-specific recombination between Bxb1 attR and attL sites, to produce Bxb1 attP and attB sites. This recombination is also unidirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of Bxb1 integrase and RDF. Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039001 and BBa_K1039001 from PB state to RL state. In concert with Bxb1 RDF (BBa_K1039006), Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039005 from RL state to PB state.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
    Illegal XhoI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1253
    Illegal AgeI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


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