Part:BBa_K1039023

Bxb1 integrase (BBa_K1039003) and lacI repressor (BBa_Q04121) are under the constitutive strong promoter BBa_J23119. Key (BBa_I714036) is under the lac inducible promoter BBa_R0011. BBa_B0015, the transcriptional terminator, is after every Bxb1 integrase and lacI repressor cassette.

Bxb1 integrase with ribosome binding sites associated with the cis-repressive RNA “lock” (BBa_J01010), so translation is inhibited. The trans-activating RNA “key” can displace the lock and allows translation.