Coding

Part:BBa_K1039013

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-11)


PhiC31 Recombination Directionality Factor

This is the recombination directionality factor (RDF) that acts with PhiC31 serine integrase. It reverses the recombination directionality of PhiC31 integrase (BBa_K1039012)

In concert with PhiC31 RDF, PhiC31 integrase (BBa_K1039012) catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF.

In concert with PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches (BBa_K1039010 and BBa_K1039011) from RL state to PB state.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 195
    Illegal SapI site found at 417
    Illegal SapI site found at 543


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Categories
Parameters
None