Part:BBa_K1037002

Sbm

methylmalonyl-CoA mutase;encodes methylmalonyl-CoA epimerase which catalyzes the reversible reaction of succinyl-CoA and methylmalonyl-CoA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 984
  Illegal BamHI site found at 1180
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 514
  

1: we firstly send our plasmid to company for gene sequence. And do sequence alignment with data from NCBI to confirm that there is no other mutant and ORF is right.
data link of NCBI:
http://www.ncbi.nlm.nih.gov/gene/945576

2. We than induce the cell transformed with plasmid to see ygfD expression. Follow is the photo of SDS-PAGE gel analysis.

3.To prove the function of the biobrick further! We do fermentation and use HPLC to measure the increase of propionate.Follow is the results.

Conclusion : with all these result, we proved that our biobrick is well constructed . We can see both the protein expressed and prove the function of the protein.