Composite
Part:BBa_K1036005
Designed by: Zhaopeng Cheng, Xiyu Wu Group: iGEM13_XMU-China (2013-09-09)
sfgfp (with LVA-tag) under plux R control
Description
This part consists of GFP variant Superfolder GFP with a LAV-tag and a quorum sensing promoter controlled luxI protein gene. Superfolder GFP with a LAV-tag is introduced in Part:BBa_K1036004, and quorum sensing mechanism is described in Part:BBa_K1036003.
Experimental Data
Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036005 which was without lux promoter and BBa_K1036006 which was under lux promoter control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2737
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
Illegal BsaI.rc site found at 2017
Illegal SapI.rc site found at 31
[edit]
Categories
Parameters
None |