Part:BBa_K1033990

Zeocin resistance cassette

Zeocin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.

Usage and Biology

The antibiotic bleomycin, a strong DNA cutting agent, is naturally produced by actinomycetes which have developed a resistance mechanism against it. This resistance protein works by sequestering of the antibiotic. E coli expressing this cassette can grow on 25 µg/ml zeocin.

References

http://www.ncbi.nlm.nih.gov/protein/6729924

http://www.rcsb.org/pdb/explore/explore.do?structureId=1BYL

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC395119/

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]