Part:BBa_K1033221
Promoter CP11
CP11 is the weakest promoter in the CP collection with the strength of 0,44 compared to the promoter J23101 in standard parts (J23101 was used as reference and its strength were put to 1).
Above you can see a diagram of all the CP promoters strength relative to J23101 [RPU]. The CP promoter parts is a collection of constitutive promoters characterized in both E. coli and Lactococcus lactis by Jensen and Hammer (1998). In E. coli it has also been characterized by us in a fluorescence-activated cell sorting machine (FACS) with BFP using the constitutive promoter J23101 in the standard parts as a reference.
These promoters have further been used successfully in several parts in our project, including BBa_K1033000 (TAL), BBa_K1033002 (STS) and BBa_K1033281 (amilCP).
In the article by Jensen and Hammer they stated that these promoters were based on the consensus sequence of promoters in gram-positive bacterium L. lactis, though there are no obvious reasons why they shouldn’t work in other organisms. Jensen and Hammer characterized them in both L. lactis and gram-negative bacterium E. coli using the reporter gene lacLM. This indicates that there is a possibility that these promoters are near universally applicable to prokaryotic organisms in general.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//collections/probiotics/control
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