Coding

Part:BBa_K1033111

Designed by: Malin Borg, Magnus Bergman, Thorstein Olaufsson, Niclas Sandberg, Nils Anlind, Pontus Danielsson, Alexander Wirtanen, Christoffer Frisk   Group: iGEM13_Uppsala   (2013-09-01)

Zeaxanthin 7,8-cleavage dioxygenase (ZCD-His) with His tag

Cleaves zeaxanthin to begin the formation of saffron metabolites such as picrocrocin, crocin and safranal. This part is from a synthetic construct based on the gene from "Crocus Sativus". The protein expressed from this gene has been shown to form insoluable inclusion bodies in the cytoplasm of bacteria. These inclusion bodies form aggregates that inhibit cell growth. Therefore, it is recommended to increase th soluability of the enzyme, for example by fusing it with a histidine patch thioredoxin.

The his-tag (6xHis) was added to facilitate characterization of the construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None