Zeaxanthin 7,8-cleavage dioxygenase (ZCD)
Cleaves zeaxanthin to begin the formation of saffron metabolites such as picrocrocin, crocin and safranal.
This part is from a synthetic construc based on the gene from "Crocus Sativus". The protein expressed from this gene has been shown to form insoluable inclusion bodies in the cytoplasm of bacteria. These inclusion bodies form aggregates that inhibit cell growth. Therefore, it is recommended to increase th soluability of the enzyme, for example by fusing it with a histidine patch thioredoxin.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 774
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC