Coding

Part:BBa_K1031112

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-09)

J23113-CapR-Terminator


Characterization

In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23113, coding sequence of CapR and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Po/J23113-CapR. The orange arrowheads represent promoters, RBSs are shown as agreen ovals, and the red circles stand for B0015 terminator.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508



[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/pputida
//function/regulation/transcriptional
//terminator/double
Parameters
positive_regulators