Part:BBa_K1031019

NahR-Psal-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter

Characteristics

K1031019 is designed to test the activation effect of φR73δ to hybrid promoter. It is composed of NahR, Psal promoter which is activated by NahR when exposed to its' inducer salicylic acid, RBS B0034, RiboJ and φR73δ.

Fig 1 Construction of activation circuit of Band-pass Filter. The combination of φR73δ with hybrid promoter and sfGFP could test if φR73δ really works as an activator.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 786
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 77
  Illegal NgoMIV site found at 618
  
• 1000
  COMPATIBLE WITH RFC[1000]

Data shown

We had proved the activation effect of φR73δ to hybrid promoter. This activation circuit is exposed to the typical inducer of NahR, 4-MeSaA, and the expression level of φR73δ is controlled by changing the inducer's concentration. The activation effect of φR73δ to hybrid promoter is shown via induction ratio calculated from fluorescence intensity.

Fig 2 Effect of activation circuit of Band-pass Filter. Horizontal axis stands for different concentrations of NahR inducer 4-MeSaA. Vertical axis represents induction ratio. From Fig 2 it is obvious that φR74δ indeed activates the hybrid promoter, thus initiates the expression of sfGFP.