Tag
Si4

Part:BBa_K1028000

Designed by: Oleg Karpov, Joseph Beton   Group: iGEM13_Leeds   (2013-08-30)

Silica binding peptide

Introduction

This BioBrick is a short peptide of 15 amino acids designed to act as a tag binding with high affinity to silica and silica-like particles. As a tag it can easily be added to the C-terminus of proteins using molecular biology techniques allowing the peptide to assist with purification or to serve other experimental processes related to silica binding.

Figure.1 Illustrated schematic of the Si4 Binding Peptide binding with a silica microsphere.


This part was originally designed by the Leeds 2013 iGEM team to bind with silica microspheres, designed to act as pathogen surrogates, when presented on the exterior of the plasma membrane via fusion with Ice Nucleation Protein.

Usage and Experimental Data

The experimental data acquired regarding this part is shown on the page for its sister part BBa_K1028001. The Si4 Binding Peptide was tested in unison with INP as an INP.Si4 complex to allow expression in the extracellular space via the plasma membrane.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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