Coding

Part:BBa_K1027001

Designed by: Robert Harrison, Lorna Hepworth, Vun Hiang Tan   Group: iGEM13_Manchester   (2013-09-19)

Delta 9 Desaturase, optimised for expression in Escherichia coli

This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesis of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 447
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aDelta 9 Desaturase, optimised for expression in Escherichia coli