Regulatory

Part:BBa_K1026009

Designed by: Hongyi WU   Group: iGEM13_SJTU-BioX-Shanghai   (2013-09-27)


A rationally designed promoter negative regulated by CcaR

By fusing G-box, a region that CcaR binds, with the constitutive promoter, BBa_J23107, we devised a promoter that is constitutive without active CcaR (the transcription factor in green light sensor system, CcaS-CcaR-PcpcG2), but be inhibited when CcaR is activated by green light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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