Reporter

Part:BBa_K1024001

Designed by: Sun Xiaochen   Group: iGEM13_Tsinghua   (2013-09-09)

Lux Promoter in Yeast (pLux+Cyc Promoter+mCherry)

Part Name: BBa_K1024001

Short Description: Lux Promoter in Yeast (pLux+Cyc Promoter+mCherry)

Part Type: Reporter

Design: We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcriptional regulated promoter in quorum sensing system, Plux promoter (BBa_R0062), by adding cyc100 mini promoter downstream of the Plux promoter (BBa_K1024001).

Tsinghua-BB-0000.png

Figure 1. The principle of quorum sensing
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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