Reporter

Part:BBa_K1023017

Designed by: Michiel Karrenbelt   Group: iGEM13_Wageningen_UR   (2013-12-19)


eForRed Chromoprotein

We synthesized and via Gibson assembly put together a chromoprotein encoding gene (eforRed) which was codon-optimized for Aspergillus niger. A mitochondrial retention signal was added to this codon-optimized chromoprotein encoding gene for targeted organelle localization. There is a Nsi restriction site in the middle that allows one to cleave of the signal peptide if required. 5'-signal seq - Nsi1 - eforRed-3'

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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