Composite
Part:BBa_K1022124
Designed by: Maithili Krishnan Group: iGEM13_TU-Delft (2013-09-20)
pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lysis
This biobrick is the one of the three final constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pT7 along with a His6 SUMO tagged peptide followed by a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 46
Illegal NheI site found at 2361
Illegal NheI site found at 4456
Illegal NheI site found at 4479
Illegal NotI site found at 3057 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 170
Illegal BamHI site found at 3026
Illegal XhoI site found at 3035
Illegal XhoI site found at 3066 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 355
Illegal AgeI site found at 4061
Illegal AgeI site found at 4131 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 4712
Information
This part is one of the final constructs designed in the project [http://2013.igem.org/Team:TU-Delft/Project Peptidor] by Team TU Delft
Please visit [http://2013.igem.org/Team:TU-Delft iGEM 2013 Team TU Delft] for more
[edit]
Categories
Parameters
None |