Composite

Part:BBa_K102142

Designed by: Wayne Materi   Group: iGEM08_Alberta_NINT   (2008-10-27)


TA14In anti-sense input in test harness

The input test harness provides a means to input one or more anti-sense RNA input signals to a T/AasL circuit. The test harness consists of an Arabinose cassette (AraC- PBad) which produces the anti-sense RNA input transcripts. Each input signal is flanked by a pair of hammerhead ribozymes to cleave the actual anti-sense signal from the rest of the transcript so that it may freely interact with (disrupt) its target gate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None