Reporter

Part:BBa_K1021021

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)

PT7+mRFP

The gene for monomeric red fluorescent protein sits downstream of the T7 promoter for expression with T7 RNA Polymerase. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation.

Usage and Biology

  • This part was constructed from BBa_I712074 and BBa_E1010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None