Coding

Part:BBa_K1021002

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-08-30)

hph (hygromycin phosphotransferase)

The hph gene encodes hygromycin phosphotransferase, which confers resistance to hygromycin.

Usage and Biology

  • Cornell iGEM demonstrated the functionality of this part via transformation (in vector pHPVEL1) into C. heterosporus. The fungal transformants grew in the presence of hygromycin:

PHG.JPG

  • This part has been used in the construction of composite and generator parts BBa_K1021028, BBa_K1021030, BBa_K1021033, and BBa_K1021034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None