Part:BBa_K1020008
alkane producing module
This part is composed of genes encoding two enzymes,NPDC and AAR. Both are key enzymes in the production of fungible hydrocarbon biofuels, for they play important roles in turning fatty acyl-ACPs into alkanes.Different from BBa_K590025 given by Washington iGEM 2010, we have done the coden optimization for ''E.coli''.And the sequence is completely different.
We constructed 3 strains to characterize the productivity of intracellular alkane:
strain1:No.53：producing module (constitutive promoter J23100)+ Alk-Sensor device
strain2:No.54：producing module (constitutive promoter J23114) + Alk-Sensor device
strain3:No.55：Null+ Alk-Sensor device
The promoter strength of No.54 is stronger than that of No.53. Theorectically, alkane productivity of No.53 and No.54 will be different, and intracellular alkane concentration of the above 3 strains will be different-- No.54 the highest, No.53 lower and No.55 the lowest.
The 3 strains are cultured in 3ml M9 medium for about 24 hours.And the vertical coordinate of picture below is fluorescent indensity per OD.
The fluorescent indensity of No.54 is the stronger than No.53, which indirectly proves that this module produce alkanes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 48
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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