Part:BBa_K1017811
Pcons+rRBS-2+mRFP+J61048
Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. We made this biobrick in order to test whether our self-designed rRBS-2 really works. Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034).
In the figure, we can see that B0034 is the strongest RBS, and our self-designed rRBS-2 is just behind it. B0030 and B0032 are at the third and the forth place. This result indicates that the rRBS-2 really works and performs well.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 784
Illegal AgeI site found at 625
Illegal AgeI site found at 737 - 1000COMPATIBLE WITH RFC[1000]
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