Device

Part:BBa_K1015017

Designed by: Kento Tominaga   Group: iGEM13_TMU-Tokyo   (2013-09-16)

FRT+Ap(for RED system)

This is genomerinsertion part.


Results of agarose gel electrophoresis

TMU-Tokyo_0902.jpg"
Figure. The left is molecular weight marker which is λ-hindⅢ. 1: PterR1, 2: PtetR2, 3: FLP, 4: Sm, 5: araC-P, 6: hbiF, 7: Tc, 8: LacI, 9: FRT-Cm, 10: FRT-Ap
The 10th lene is K_1015017.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 116
    Illegal XbaI site found at 34
    Illegal XbaI site found at 171
    Illegal XbaI site found at 1144
    Illegal XbaI site found at 2113
    Illegal PstI site found at 183
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 116
    Illegal NheI site found at 444
    Illegal PstI site found at 183
    Illegal NotI site found at 846
    Illegal NotI site found at 876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 116
    Illegal BamHI site found at 137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 116
    Illegal XbaI site found at 34
    Illegal XbaI site found at 171
    Illegal XbaI site found at 1144
    Illegal XbaI site found at 2113
    Illegal PstI site found at 183
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 116
    Illegal XbaI site found at 34
    Illegal XbaI site found at 171
    Illegal XbaI site found at 1144
    Illegal XbaI site found at 2113
    Illegal PstI site found at 183
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 109


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Categories
Parameters
None