Part:BBa_K1015015

araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)

With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.


Results of agarose gel electrophoresis


Fig.1 This result is 2nd PCR of overlap extension PCR.
From left to right, this results is molecular weight marker which is λ-hindⅢ, K1015014①, K1015014②, K1015015①, K1015015②.
K1015015① is araC(K1015004)-pBAD(K1015005) and hbiF(K880000). And K1015015② is hbiF(K880000), Tetracyclin resistance(K1015006) and araC homology sequence(BBa_K1015007).



Fig.2 This result is 3rd PCR of overlap extension PCR.
The left is molecular weight marker which is λ-hindⅢ. The right is K1015015.


Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2912
  Illegal XbaI site found at 2226
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2912
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2912
  Illegal BamHI site found at 1193
  Illegal BamHI site found at 1518
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2912
  Illegal XbaI site found at 2226
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2912
  Illegal XbaI site found at 2226
  Illegal AgeI site found at 1028
  Illegal AgeI site found at 1353
  Illegal AgeI site found at 3348
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1010
  Illegal SapI site found at 1335