Coding

Part:BBa_K1015006

Designed by: Eri Sakurai   Group: iGEM13_TMU-Tokyo   (2013-09-16)

TetA gene from E.coli


Tetracycline is a broad-spectrum polyketide antibiotic produced by the Streptomyces genus of Actinobacteria, indicated for use against many bacterial infections. It is a protein synthesis inhibitor. This part includes a gene which has resistance to tetracyclin.

In our project, we use this part as a marker to select colonies of E.coli which succeeded in homologous recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 740
    Illegal XbaI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 740
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 740
    Illegal XbaI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 740
    Illegal XbaI site found at 54
    Illegal AgeI site found at 1176
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None