Composite

Part:BBa_K1014999

Designed by: Xiangmiao Zeng   Group: iGEM13_HIT-Harbin   (2013-09-02)

Biological proportional operational Mu-circuit(1)

Biological proportional operational Mu-circuit 1

The composition of B-POM is that hrpR's promoter depends on the input IPTG, but hrpS’promoter is always Ptet and tet owns PhrpL, while the output gene follows tet and shares PhrpL. Once the input is sensed, the input promoter triggers hrpR's transcription. The activity of Ptet is constitutive, which means HrpS protein is ample. As HrpR accumulates, HrpR binds to HrpS and form HrpRS which then triggers PhrpL, and tetR and output begin to accumulate. tetR can inhibit Ptet. As a feedback, HrpS and HrpRS will decrease. PhrpL will be of lower activity, so the amount of tetR and the level of output will decline. The decrease of tetR will enhance the hrpS' expression. All these construct a feedback cycle. Finally, the output will stabilize and be in a certain proportion with the input. By manipulating the RBS of hrpR, hrpS, tetR and output gene, we can control input-output proportion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 205
    Illegal AgeI site found at 211
    Illegal AgeI site found at 259
    Illegal AgeI site found at 825
    Illegal AgeI site found at 850
    Illegal AgeI site found at 3039
    Illegal AgeI site found at 3151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None