Regulatory
Part:BBa_K101018
Designed by: Karl Petersen Group: iGEM08_Minnesota (2008-10-28)
MioC Promoter (regulating tetR)
The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109).
This promoter is upstream of a tetR (BBa_I15040), which is repressed by the binding of DNAa during initiation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal SpeI site found at 314 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 314
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal SpeI site found at 314 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 314 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |