Part:BBa_K101015

Standard Promoter with RBS, GFP and terminator

Standard promoter (J61002) cloned with E0240 (RBS-GFP-terminators) for promoter strength measurement.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 706

The device was used to compare promoter strength. Standard reference promoter (BBa_J23101) was cloned upstream to GFP. The input is aTc and output is green fluorescence. Fluorescence was measured in TOP 10 cells.

Input: aTc

Output: Green Fluorescence (501-520 nm)

Experiment

Characteristic

Value

Promoter Activity in TOP 10 cells

Maximum Fluorescence

586246.0 (Normalized fluorescence at OD₆₀₀~1.0)3 hours after the growth.

Growth of culture in LB media

Doubling time of the recombinant cells

~ 25 Minutes

Compatibility
Devices: Device has been shown to work with BBa_E0040, Bba_B0010, and Bba_B0010

Plasmids: Device has been shown to work on pSB3K3