Composite

Part:BBa_K101010

Designed by: Bennett Swiniarski   Group: iGEM08_Minnesota   (2008-07-28)


Dual-Repressed Promoter for p22 cII and TetR with RBS attached

This composite part consists of a dually-repressed promoter with a medium strength RBS attached. The promoter is repressed by either p22 cII or TetR proteins. The medium strength RBS was attached to make this promoter easier to use with any protein that may need to be attached to it. When the RBS is already on the promoter, a simpler ligation can occur that attaches the protein to this promoter, instead of having to attach a small RBS before this ligation can occur.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None