Composite

Part:BBa_K101007

Designed by: Sarah Hendrickson   Group: iGEM08_Minnesota   (2008-07-28)

TetR and p22 cII Dual Promoter Driving RFP

This is a construct composed of a dually-repressed promoter, an RBS of medium strength, the reporter protein RFP, and a single, well-characterized terminator. The promoter is repressed in the presence of TetR and/or p22 cII, and ideally allows no expression of the reporter RFP. The RFP used is BioBrick part J06504, a monomeric RFP which has been optimized for bacteria.

The presence of RFP makes this construct a useful reporter in E.coli systems in which expression levels of either TetR and/or p22 cII need to be examined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None