Part:BBa_K101006

Construct with TetR/p22mnt promoter, RBS, RFP, Terminator

The promoter contains binding sites for both TetR and p22mnt. RBS is the ribosomal binding site. RFP is the red fluorescent protein, which is a reporter gene. The terminator will terminate transcription.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 42
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

The device has a dual promoter which is repressed by tetR and p22mnt. The input is aTc and output is fluorescence as gene for mRFP (BBa_J61002) is cloned downstream to the dually repressed promoter.

Input: aTc

Output: Red Fluorescence

Experiment	Characteristic	Value
Promoter Activity in TOP 10 cells	Maximum Fluorescence	277006.0 (Normalized fluorescence at OD₆₀₀~1.0)3 hours after the growth.
Growth of culture in LB media	Doubling time of the recombinant cells	~ 25 Minutes
Response time (after addition of IPTG)	~20 Minutes

Compatibility
Chassis: Device has been shown to work in BBa_J61002, BBa_B0034, and BBa_B0015

Plasmids: Device has been shown to work in pSB3K3