Part:BBa_K1010002

AraC/XylS family regulatory protein(NphR)

AraC/XylS family regulatory protein(NphR) and PNP positive regulatory protein, which is necessary for promoter nphA1 (BBa_K1010001).

Electrophoretogram of nphR

Promoter nphA1's Transcription without nphR

Promoter nphA1's Transcription with nphR

Acknowledgement

Thanks for Dr. Masahiro Takeo, who offered bacterium Rhodococcus sp. Strain PN1 and plasmids.

References

Takeo, Masahiro, Masumi Murakami, Sanae Niihara, Kenta Yamamoto, Munehiro Nishimura, Dai-ichiro Kato, and Seiji Negoro. "Mechanism of 4-nitrophenol oxidation in Rhodococcus sp. Strain PN1: characterization of the two-component 4-nitrophenol hydroxylase and regulation of its expression." Journal of bacteriology 190, no. 22 (2008): 7367-7374.

Yamamoto, Kenta, Munehiro Nishimura, Dai-ichiro Kato, Masahiro Takeo, and Seiji Negoro. "Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1." Journal of bioscience and bioengineering 111, no. 6 (2011): 687-694.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 850
  Illegal XhoI site found at 966
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 258
  Illegal NgoMIV site found at 913
  
• 1000
  COMPATIBLE WITH RFC[1000]