Part:BBa_K1010001

RBS (B0034) + NphR (PNP-responsive positive regulator) + Terminator (B0015)

This part can express NphR, an araC-type PNP-responsive positive regulator.

Protein nphR is necessary for promoter nphA1 (BBa_K1010001).

Promoter nphA1's Transcription without nphR

Promoter nphA1's Transcription with nphR

Acknowledgement

Thanks for Dr. Masahiro Takeo, who offered bacterium Rhodococcus sp. Strain PN1 and plasmids.

References

Takeo, Masahiro, Masumi Murakami, Sanae Niihara, Kenta Yamamoto, Munehiro Nishimura, Dai-ichiro Kato, and Seiji Negoro. "Mechanism of 4-nitrophenol oxidation in Rhodococcus sp. Strain PN1: characterization of the two-component 4-nitrophenol hydroxylase and regulation of its expression." Journal of bacteriology 190, no. 22 (2008): 7367-7374.

Yamamoto, Kenta, Munehiro Nishimura, Dai-ichiro Kato, Masahiro Takeo, and Seiji Negoro. "Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1." Journal of bioscience and bioengineering 111, no. 6 (2011): 687-694.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 862
  Illegal XhoI site found at 978
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 270
  Illegal NgoMIV site found at 925
  
• 1000
  COMPATIBLE WITH RFC[1000]