Intermediate
"Scissors"

Part:BBa_K1001755

Designed by: Vansh Singh   Group: iGEM13_The_Agency_Escondido   (2013-05-02)


AraC generator then Lambda promoter controlling RFP production, "Scissors"

This is a project specific part. This sequence contains an AraC coding gene (BBa_K228032) upstream of a double terminator behind a Red Flourescent Protein and Ribosome Binding Site behind the modified Lambda Prm Promoter(BBa_K1001754). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1629
    Illegal AgeI site found at 1741
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
negative_regulators1, 434 cl
positive_regulators1, Lamda Repressor cl