Intermediate
"Part Y"

Part:BBa_K1001754

Designed by: Vansh Singh   Group: iGEM13_The_Agency_Escondido   (2013-05-02)


Terminator RFP and RBS behind Lamda Prm Promoter

This sequence contains double terminator(BBa_B0015) behind a Red Flourescent Protein and Ribosome Binding Site behind the modified Lambda Prm Promoter(BBa_K1001753). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed. Use this BioBrick if your team wishes to have the inducible Lamda Prm promoter to control the expression of an RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 663
    Illegal AgeI site found at 775
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
negative_regulators1, 434 cl
positive_regulators1, Lamda Repressor cl