Intermediate
"Part X"
Part:BBa_K1001753
Designed by: Vansh Singh Group: iGEM13_The_Agency_Escondido (2013-05-02)
RFP and RBS behind Lamda Prm Promoter
This sequence contains a Red Flourescent Protein and Ribosome Binding Site (BBa_S03873) behind the modified Lambda Prm Promoter(BBa_I12006). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed. Use this BioBrick if your team wishes to have the inducible Lamda Prm promoter to control the expression of an RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 663
Illegal AgeI site found at 775 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
abs | |
biology | |
emit | 607 |
excitation | 584 |
excite | 584 |
lum | |
negative_regulators | 1, 434 cl |
positive_regulators | 1, Lamda Repressor cl |
protein | mRFP1 |
tag | None |