Measurement
P1+E0240

Part:BBa_K100005

Designed by: Garrett Tobin   Group: iGEM08_PennState   (2008-08-18)


Edited Xylose Regulated Bi-Directional Operator 1 + GFP

This part is the Edited Xylose Regulated Bi-Directional Operator 1 (BBa_K100001) followed by an RBS GFP Termonator (BBa_E0240). This composite part measures activation of the promoter, used to determining if xylose-glucose diauxie is eliminated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 974


[edit]
Categories
Parameters
None