Generator
Part:BBa_K098994
Designed by: Harvard iGEM 08 Group: iGEM08_Harvard (2008-10-26)
mtrB with RBS and terminator
- WARNING: mtrB is toxic to DH5alpha (likely extends to other E. coli strains)
- may have point mutations
- thorough sequencing is advised before use
- see details under Part Design
Usage and Biology
- outer membrane protein precursor MtrB from Shewanella oneidensis MR-1
- required for normal electron transport to external environment under anaerobic conditions
- allows cells to use external substrates as final electron acceptor when oxygen is absent
- not needed in wild-type cells
- mtrB deficient cells can survive under anaerobic respiration
- required for normal electron transport to external environment under anaerobic conditions
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |