Generator

Part:BBa_K098994

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)

mtrB with RBS and terminator

  • WARNING: mtrB is toxic to DH5alpha (likely extends to other E. coli strains)
  • may have point mutations
    • thorough sequencing is advised before use
    • see details under Part Design


Usage and Biology

  • outer membrane protein precursor MtrB from Shewanella oneidensis MR-1
    • required for normal electron transport to external environment under anaerobic conditions
      • allows cells to use external substrates as final electron acceptor when oxygen is absent
    • not needed in wild-type cells
    • mtrB deficient cells can survive under anaerobic respiration


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None