Generator

Part:BBa_K098990

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)

mtrB, low expression

  • outer membrane protein precursor MtrB from Shewanella oneidensis MR-1
    • required for external electron transport
  • expressed under weak E. coli promoter
  • WARNING: mtrB is toxic to DH5alpha (likely extends to other strains)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None