Composite

Part:BBa_K098988

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)


temperature sensitive cI inducible system with GFP reporter and high promoter

This is a temperature sensitive cI inducible system driven by a high promoter (BBa_J23114) for the repressor and with a GFP reporter. It can be used to regulate the expression of GFP using heat.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1600


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