Generator

Part:BBa_K098984

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)

lac inducible mtrB (high expression of QPI)

  • WARNING: toxic to DH5alpha (likely extends to other strains without constitutive lacI expression)
  • QPI driven by strong promoter
  • mtrB expression default off
  • induced by IPTG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None