Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K094171

Designed by: Li Xinran   Group: iGEM08_iHKU   (2008-10-14)


pTetR-rbs-lacZ alpha

lacZ alpha fragment with a Tet promoter. This part is without a terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2058
    Illegal BamHI site found at 2397
    Illegal XhoI site found at 2448
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097


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Parameters
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