Coding

Part:BBa_K094113

Designed by: lacZ   Group: iGEM08_iHKU   (2008-10-14)


lacZ

the cheY* gene is installed in front of a lacI operon. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This lacI operon contains a mutated LacIq promoter which enables high level of transcription. The cheY* gene is without a promoter, rbs or terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 109
    Illegal XbaI site found at 154
    Illegal SpeI site found at 84
    Illegal PstI site found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 109
    Illegal SpeI site found at 84
    Illegal PstI site found at 114
    Illegal NotI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 109
    Illegal BamHI site found at 78
    Illegal XhoI site found at 142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 109
    Illegal XbaI site found at 154
    Illegal SpeI site found at 84
    Illegal PstI site found at 114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 109
    Illegal XbaI site found at 154
    Illegal SpeI site found at 84
    Illegal PstI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None