Measurement

Part:BBa_K094105

Designed by: Shi Lei   Group: iGEM08_iHKU   (2008-10-14)


plambda P(O-R12)-rbs-cheZ-rbs-mRFP1-terminator

the cheZ gene is installed in front of a lacI operon. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This lacI operon contains a mutated LacIq promoter which enables high level of transcription. The cheZ gene is without a promoter, rbs or terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 92
    Illegal NheI site found at 772
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 92
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 92
    Illegal AgeI site found at 1402
    Illegal AgeI site found at 1514
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None