Generator
pR-RFP

Part:BBa_K093010

Designed by: Danielle Nash   Group: iGEM08_Waterloo   (2008-10-29)

lambda pR-RFP generator

This part Contains the lambda Pr promoter, BBa_R0051, denoted POcI, since it contains the operator for lambda cI repressor. After which, it contains the Elowitz RBS, BBa_B0034, and RFP, BBa_E1010

This part has been tested in the absence of the repressor, with high expression of the reporter when plated on solid media and in liquid culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None