Part:BBa_K093000

pRecA with LexA binding site

This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1.

Preliminary testing has been done to characterize this promoter using RFP as a reporter (K093009). The promoter is active, demonstrating minimal leaky expression compared to the constitutive promoter pconst (J23118). The ability to upregulate has been tested by exposing culture to UV to induce crosslinking, but more trials must be conducted to optimize conditions. A crosslinking agent such as mitomycin C could also be used to test induction.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]