Project

Part:BBa_K092901

Designed by: EPF-Lausanne Team 2008   Group: iGEM08_EPF-Lausanne   (2008-10-22)


RhlR, PrhlR + LacI modified , PLac + TetR, pTetR + RFP and LuxI

Sequence containing : - RhlR controlled by a constitutive promoter - LacI modified with a RhlR promoter - TetR controlled by a Lac Promoter - RFP controlled by a TetR promoter - LuxI is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779


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Categories
Parameters
None